Posted 26 February 2009 - 08:24 AM
I was in doubt as to why my protocol says add sterilized MgCl2 separately and JUST BEFORE USE.
Does that mean that this medium is good for one time use only?
I make SOC stock and use it for a long time, so I don't think this would be the case, but I don't get the "JUST BEFORE USE" part.
Posted 26 February 2009 - 08:52 AM
Provided you sterile technique is good, and with the help a refrigerator, you can use the same bottle of medium several times.
MgCl2 solution has to be added after the autoclaving. Due to the amount of MgCl added to the medium, the Mg ions it will form a precipitate if autoclaved. And once Mg percipitate forms it is hard to redissolve.
Posted 26 February 2009 - 02:23 PM
Posted 26 February 2009 - 07:56 PM
Posted 28 February 2009 - 08:19 AM