Posted 26 February 2009 - 09:24 AM
I used the protocol for preparing RNase A from Sambrook, which seems to work fine. An important step is to boil the RNase before you use it. The protocol is as follow:
1. dissolve RNase in 0.01M sodium acetate
2. heat to 100C for 15min, cool down at room temperature
3. adjust pH by adding 0.1vol of 1M Tris-Cl (ph 7.4)
4. dispense in aliquots and store at -20C
The optimum working range for RNase A is between pH 7 and 8 (I think).
If that protocol doesn't work, it might be worth to buy pre-made RNase A from Invitrogen.
"You can give somebody a book on 'How to ride bike' and then test that person on that knowledge. Even if that person gets an "A", it doesn't mean that he or she can ride a bike."
---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V