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Guanidinium thiocyanate and SDS-PAGE


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#1 Hanan

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Posted 26 February 2009 - 01:25 AM

Hi,
I'm searching information about guanidinium thiocyanate and how it can be applied in SDS-PAGE to reduce protein-protein interactions (my protein sample is an aggregated form).
Thanks for any help in advance
Hanan Hilmi

#2 AquaPlasmid

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Posted 26 February 2009 - 10:06 AM

GuCN and SDS are not compatible.

#3 Hanan

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Posted 27 February 2009 - 12:16 AM

Hi,
I'm searching information about guanidinium thiocyanate and how it can be applied in SDS-PAGE to reduce protein-protein interactions (my protein sample is an aggregated form).
Thanks for any help in advance


Hanan Hilmi

#4 Hanan

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Posted 27 February 2009 - 12:32 AM

thanks for your reply,my point is GuCN is a general protein denaturant so I can use it for my aggregated protein to reduce protein-protein interactions ,and the commonly used method is Guanidinium thiocyanate-phenol-chloroform extraction(as It is not strictly to use phenol or chloroform in extracting) and followed by SDS -PAGE!
Hanan Hilmi




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