Problem with Colony PCR
Posted 25 February 2009 - 11:05 PM
I did a colony PCR to check the plasmid and insert then on the agarose gel some samples appeared as many bands and I don't know what is the problem with the experiment.
Thank you in advance
Posted 25 February 2009 - 11:58 PM
Posted 26 February 2009 - 06:11 AM
Posted 26 February 2009 - 09:21 PM
I sent the colony PRC product for sequencing - the forward primers worked but the reverse primers didn't work for all experiments- but when I look in database I found the insert match what I look for in database.
What I did is: I pick 4 colony from the same plate but the result is very different two of them got a good bands for insert and plasmid but the others appeared as many bands( look like a ladder), I don't know what is the problem? Is it in primers or