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invitro transcription


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#1 Seifollah azadi

Seifollah azadi

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Posted 12 August 2002 - 04:49 AM

Hi
1- could any body tell me if I can see the dig labeled RNA probe on the gel
2- which kind of gel should I use, denaturing ( formaldehyde ) gel or normal agarose gel??
thank you

#2 snowman

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Posted 24 September 2002 - 05:05 AM

1- transfer the RNA in the gel to a nitrocellulose membrane (like in northern blotting) and then develop the membrane eg. with ECL reagent (like on western blot); i never tried in-gel dection.
2- agarose gel is fine, but lower resolution, acrylamide gels provide better resolution




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