lysing cells in LSB
Posted 24 February 2009 - 08:59 AM
have you ever tried lysing cells directly in LSB?
have you had luck with it?
Posted 01 March 2009 - 11:12 PM
Posted 18 March 2009 - 04:42 PM
Hi. Yup, but need to sonicate to minimize viscosity due to nuclear content.
I wondered if lysing them in 1x LSB or 5X LSB makes a huge difference.
I'm really trying to preserve phospho-ERK in my samples and I wondered if I should use RIPA or stay with LSB but a phosphatase inhibitor.