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lysing cells in LSB


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#1 bluelady09

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Posted 24 February 2009 - 08:59 AM

hey guys,

have you ever tried lysing cells directly in LSB?
have you had luck with it?
just curious.

#2 lsek

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Posted 01 March 2009 - 11:12 PM

Hi. Yup, but need to sonicate to minimize viscosity due to nuclear content.

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#3 bluelady09

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Posted 18 March 2009 - 04:42 PM

Hi. Yup, but need to sonicate to minimize viscosity due to nuclear content.


I wondered if lysing them in 1x LSB or 5X LSB makes a huge difference.
I'm really trying to preserve phospho-ERK in my samples and I wondered if I should use RIPA or stay with LSB but a phosphatase inhibitor.




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