hey guys,
have you ever tried lysing cells directly in LSB?
have you had luck with it?
just curious.
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#2
lsek
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Posted 01 March 2009 - 11:12 PM
Hi. Yup, but need to sonicate to minimize viscosity due to nuclear content.
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#3
bluelady09
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Posted 18 March 2009 - 04:42 PM
lsek, on Mar 2 2009, 12:12 AM, said:
Hi. Yup, but need to sonicate to minimize viscosity due to nuclear content.
I wondered if lysing them in 1x LSB or 5X LSB makes a huge difference.
I'm really trying to preserve phospho-ERK in my samples and I wondered if I should use RIPA or stay with LSB but a phosphatase inhibitor.
