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starting total RNA concentration for miRNA analysis

Started by flashboy, Feb 24 2009 05:13 AM

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#1 flashboy

flashboy

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Posted 24 February 2009 - 05:13 AM

Hi all,

If i wanted to use total RNA samples for miRNA analysis, what starting concentration should I use? the Ambion kit has a heart total RNApositive control at 25ng/ul.... Would it be bad to use a higher concentration than this?

Cheers
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