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synthetic scfv


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#1 desertrose

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Posted 24 February 2009 - 03:55 AM

Dear bioforumers
I am working in a project for construction of synthetic scfv,the Ab was tailored by the PI who is abroad now.
Please could anyone tell me how the design is made or suggest some readings or softwares.

#2 phage434

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Posted 27 February 2009 - 04:31 PM

I can send you some readings when I get into the lab. What do you have for Ab now? Is it a monoclonal? If so, then the first step would be to sequence it, presumably by reduction, trypsin digestion and mass spec. If it's polyclonal, you'll likely have to first make a monoclonal. Once you have a protein sequence, then the conversion to an ScFv is reasonably straightforward, though not guaranteed to give good results.

#3 desertrose

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Posted 28 February 2009 - 05:47 AM

Thanks Dr. my ab is monoclonal
Iam waiting for the readings

Edited by desertrose, 28 February 2009 - 05:49 AM.





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