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Dendritic_ cells


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3 replies to this topic

#1 inas

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Posted 23 February 2009 - 03:52 PM

hi I am PhD student (Immunology) i am facing problem in lab when i isolat Monocyte from human blood using CD14 beads and generate them to dendritic cells most the cells die during incubation or lose them during the washing step, i need helo in solving that problem because i dont knoe the cause of that . Please

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#2 almost a doctor

almost a doctor

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Posted 25 February 2009 - 07:40 AM

Hi inas,

could you tells us some more details. Actually, if you post your protocol we will be able to help you better.

:huh:

#3 inas

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Posted 26 February 2009 - 02:13 PM

Hi ,
My protocol is attached

Thank you for your reply

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#4 inas

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Posted 26 February 2009 - 02:25 PM

Please answer me as soon as possible, I realy need help
Regards :o




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