hi,
Is there anyway to purify an RNA probe after in vitro transcription without using tRNA as a carrier for precipitation.
vimala
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#2
anonymous
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Posted 07 February 2001 - 10:00 PM
Try using one of the many spin columns found on the market. I've found pharmacia's s-200 columns to work well.
#3
anonymous
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Posted 08 February 2001 - 10:00 PM
you can also purify you RNA transcripts using PAGE gel, cut the band and elute over night at 37C in elute buffer and use it without further treatment. Recently I used this method to prepare my RNA probe for RNase Protection analysis and it works quite well.
Good luck
#4
Noel
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Posted 20 September 2004 - 10:00 PM
| QUOTE ( @ Feb 8 2001, 11:00 PM) | |
| you can also purify you RNA transcripts using PAGE gel, cut the band and elute over night at 37C in elute buffer and use it without further treatment. Recently I used this method to prepare my RNA probe for RNase Protection analysis and it works quite well.<p>Good luck [/quote] Hi, I was just wondering if you could make available your protocol for band extraction? Thanks Noel #5
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Neutral Posted 21 September 2004 - 04:53 PM
I work with almost nothing but RNA and it is pretty easy. UV shadow or radio label your RNA cut the band out and either place it in TE and 0.1 %SDS overnight or electroelute it (can be done in dialaysis tubing and a agrose gel box). If you electroelute you will need to concentrate (butanol or microcon). If you need it to be super clean (NMR etc) then use a A25 DEAE (sigma).
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