10 replies to this topic

[stardust]

Hello,

i have a quick question regarding long term storage of antibodies. My previous supplier stated in the datasheet: mix with equal amount of 99% glycerol and freeze aliquots at -20°C. Now i ordered antibodies from sigma and they don't say anything about adding glycerol...does that mean I can just freeze the antibody in aliquots without glycerol?

Thanks Stardust

[mdfenko]

store them the way the provider recommends.

they have tested and approved the conditions.

if you store differently then you void any warranty that the company gives.

[stardust]

Ok thanks for your answer!

Stardust

[scolix]

My ex PI would always tell us to freeze antibodies at -20C no matter what the data sheet says.

[WHR]

scolix, on Feb 28 2009, 09:05 AM, said:

My ex PI would always tell us to freeze antibodies at -20C no matter what the data sheet says.

Same as mine. The company tell you to store the ab at 4C, and it is stable for months because they want to sell more.  

[Gerard]

I store antibodies as prescibed in the insert and I've never had problems before the exp.date even allmost every antibody was useable long after the given exp.date.

[stardust]

Thanks  for all your answers! I have frozen it now in aliquots at -20°C...

Stardust

[madrius1]

I would agree whit Gerard, as I stored some Abs at 4 (as recommended by the supplier) and every single one is still perfectly working, over 1 year later.

[Nrelo]

madrius1, on Mar 2 2009, 07:01 AM, said:

I would agree whit Gerard, as I stored some Abs at 4 (as recommended by the supplier) and every single one is still perfectly working, over 1 year later.

yup, our Ab remain powerful after storing at 4C for over 3 yr. The datasheet suggest us to store at 4C for no more than 1 yr. As a matter of fact, those suppliers want to sell as much Ab as they can.

[butterfly]

Nrelo, on Mar 3 2009, 05:11 AM, said:

madrius1, on Mar 2 2009, 07:01 AM, said:

I would agree whit Gerard, as I stored some Abs at 4 (as recommended by the supplier) and every single one is still perfectly working, over 1 year later.

yup, our Ab remain powerful after storing at 4C for over 3 yr. The datasheet suggest us to store at 4C for no more than 1 yr. As a matter of fact, those suppliers want to sell as much Ab as they can.

I cannot not agree with you that always store at -20. If the data sheet said donot freeze the antibody, you still freez it anyway? I believe it depend on how the manufactory purified the antibody, especially the storage buffer. I think the smart way is follow what they suggested.

[Marvilla]

Maybe in both storage temperatures antibodies will work, but in one of them the storage is going to be optimal. If the antibody is disolved in glycerol solution then at -20 it won't be frost and it can be used directly.