I seem to be getting a low yield (15ng/ul) when trying to purify my DNA from 1% agarose gels with the Qiagen PCR Purification kit. I have two vectors - in two seperate reactions 1 has been cut with AflII & the other with Sal I & ran on a gel. Got strong band for each cut vector on their respective gels but when i cut out & purify the yield seems to be really low. Any suggestions?
Edited by moorele, 23 February 2009 - 08:35 AM.