Posted 25 February 2009 - 04:12 AM
Thank you all for your answers…if you can think of any other way or trick, please let me know.
I know it sound like a stupid mistake-to mix tubes, but…it can happen to anyone. Unfortunately, it happened to my lab partner, and he feels pretty bad, so I*m not trying to make him feel even worse, instead we look around for a solution.
Here are some of the answers to the questions that you have asked me:
1. Do you know anything about the origins of the tissue? Do they come from the same individual? Tissue=brain tumor (metastasis to be precisely, primary tumor was found in lungs). Tissue and blood come from the same person (male)
2. what type of protocol was use for each extraction? Method of isolation by salting out the proteins is faster, less aggressive, and uses less dangerous chemicals than the method of phenol and chloroform. We did comparison of these two methods-and statistic analysis showed that the two methods gave equally good results. So, our lab decided to use method of salting out, because of the reasons above mentioned.
3. this a homework question? No, unfortumately, no. This is a real problem.
4. The nature of the tissue…are they methylated in different ways based on tissue specificity? I don*t know… Our lab only gets informations I already mentioned (Answer no.1).
Remark about Hb OD gave me some idea…Perhaps, simply be measuring concentration of DNA in samples we can get some indication…as you*ve stated out there is much more DNA in the tissue, so the DNA concentration of that sample should be higher…but this is not proof…
“Do not go where the path may lead, go instead where there is no path and leave a trail.”
Ralph Waldo Emerson