Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

lysis buffer to use for enzyme assay


  • Please log in to reply
1 reply to this topic

#1 BottegaVeneta

BottegaVeneta

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 22 February 2009 - 07:48 AM

hello,

i'd like to do an enzyme activity assay comparing treated/untreated cells. is there a particular buffer i should use to lyse the cells but not denature the proteins?

thank you in advance

#2 T C

T C

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 277 posts
0
Neutral

Posted 22 February 2009 - 08:15 PM

Yeah

I use 50 mM Tris pH8 with 10 % glycerol or 100 mM phosphate pH 7.2 with 10 % glycerol. Also, u may have to add other things like ATP, CoASH or NAD(P)H if yr enzyme needs it.

Best

TC




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.