distance between probe and primers in Taqman method
Posted 21 February 2009 - 11:00 AM
Posted 21 February 2009 - 12:30 PM
I don't know but I generally keep at least 7-10 nt distance on both sides, and most of my primer-probe combos have worked.
I know that Taqman probes should not overlap with the primers when doing real-time pcr, but can you please tell me how far the probe and primers could be located from each other so that the PCR is efficient?
Posted 06 April 2009 - 04:23 AM