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distance between probe and primers in Taqman method


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#1 Curtis

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Posted 21 February 2009 - 11:00 AM

I know that Taqman probes should not overlap with the primers when doing real-time pcr, but can you please tell me how far the probe and primers could be located from each other so that the PCR is efficient?

#2 TanyHark

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Posted 21 February 2009 - 12:30 PM

I know that Taqman probes should not overlap with the primers when doing real-time pcr, but can you please tell me how far the probe and primers could be located from each other so that the PCR is efficient?

I don't know but I generally keep at least 7-10 nt distance on both sides, and most of my primer-probe combos have worked.

#3 chrisbelle

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Posted 06 April 2009 - 04:23 AM

The primers should be designed as close as possible to the probe without overlapping it.
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