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Negative control problem in BSP

Started by luck, Feb 21 2009 05:28 AM

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#1 luck

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Posted 21 February 2009 - 05:28 AM

I have some question about BSP. I design a methylation primer set for detect the methylated state of a gene in Arabidopsis, but the methlation primer set is very weird.The negative control always show a ladder form,only the first 2 or 3 times I can get clean negative control.I think I didn’t contaminate the reagent. So I want to know why this happens? Is there any possibility that the primer sets form the ladder?

Thanks

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#2 microlight

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Posted 23 February 2009 - 01:44 PM

luck, on Feb 21 2009, 05:28 AM, said:

I have some question about BSP. I design a methylation primer set for detect the methylated state of a gene in Arabidopsis, but the methlation primer set is very weird.The negative control always show a ladder form,only the first 2 or 3 times I can get clean negative control.I think I didn’t contaminate the reagent. So I want to know why this happens? Is there any possibility that the primer sets form the ladder?

Thanks

Probably dimer issue