Negative control problem in BSP
Posted 21 February 2009 - 05:28 AM
Posted 23 February 2009 - 01:44 PM
I have some question about BSP. I design a methylation primer set for detect the methylated state of a gene in Arabidopsis, but the methlation primer set is very weird.The negative control always show a ladder form,only the first 2 or 3 times I can get clean negative control.I think I didnít contaminate the reagent. So I want to know why this happens? Is there any possibility that the primer sets form the ladder?
Probably dimer issue