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Histology/Paraffin Embedding


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#1 IBNAMmonkey

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Posted 20 February 2009 - 11:44 AM

I have been culturing cells in a 3-D gel and after fixing the gels in formalin, I have attempted to embed them in paraffin. When I section the paraffin, however, a common problem is that paraffin surrounding the gel is flaky and brittle. As a result, I'm left with a hole in the section where my gel should be.

I think the problem has to do with the embedding process. During embedding the gels are much cooler than the paraffin and as a result, when I initially place the gels into the paraffin, I think a layer of amorphous paraffin forms around the gel. (If you look at the finished paraffin block, the areas surrounding the gel are white paraffin).

My question is this: Is there any way to reform that amorphous region without removing/damaging the gel inside? For instance, could I heat the block up to 60 deg C and try to re-from those regions??

#2 scolix

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Posted 21 February 2009 - 07:20 AM

Speak to your histology core (the people who can adjust things to suit paraffin embedding). They are the ones who could really help you.

#3 genehunter

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Posted 22 February 2009 - 12:11 PM

maybe it is because your gel only has a few % of matrix that makes it fragile? I dont know if cryosection after OCT embedding, or plastic resin will help.




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