Whats the role of PMSF in the protein storage. What it does?????
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#2
paramyosin
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Posted 20 February 2009 - 08:08 AM
PMSF (phenylmethanesulphonylfluoride or phenylmethylsulphonyl fluoride) is a serine protease inhibitor therefore it binds specifically to the active serine residue in a serine proteases avoiding the proteolysis of these kind of proteases over your specific protein in a crude extract. It is very useful in purification procedures, but i know that it has a low half-life so you have to take this into account.
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#3
mdfenko
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Posted 20 February 2009 - 08:38 AM
it is also irreversible so the proteases that it kills stay killed. the pmsf will only need to be replenished if anything is added to (or anything grows in) the solution.
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