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How important is it to add exactly 1 volume?


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3 replies to this topic

#1 Wolverena

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Posted 20 February 2009 - 07:52 AM

I have been wondering how important is it to add exactly 1 volume of Phenol:Chloroform:Isoamyl alcohol when doing DNA extraction....for example, you have 800ul of your solution and you would add 1ml of Phenol:Chloroform (instead of 800ul), would it matter?

Edited by Wolverena, 20 February 2009 - 07:52 AM.

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#2 perneseblue

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Posted 20 February 2009 - 09:44 AM

no, the exact volume added would not matter. It is more important to phenol/chloroform extract until the interface is clear... whereby nearly all the protein in the sample has been removed.
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#3 sciencedork

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Posted 03 April 2009 - 01:07 PM

You need to have AT LEAST that much. More is fine, less is NOT

#4 chrisbelle

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Posted 06 April 2009 - 04:25 AM

They just tell you to add 1 volume so you don't overfill your eppi tube....
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