• Create Account

Submit your paper to J Biol Methods today!

# Maximal and minimal volume in T75 and T25 flask

4 replies to this topic

### #1 Biog

Biog

Enthusiast

• Active Members
• 40 posts
2
Neutral

Posted 18 February 2009 - 08:43 AM

Hi,

I have a question about the maximal and minimal volumes of culture medium to be used in a flask of T75 and T25 ?
Please, anyone knows how many ml at maximum and at minimum should I use in each flask ?

### #2 cellcounter

cellcounter

Control Panel

• Active Members
• 145 posts
3
Neutral

Posted 18 February 2009 - 11:09 AM

Hi,

I have a question about the maximal and minimal volumes of culture medium to be used in a flask of T75 and T25 ?
Please, anyone knows how many ml at maximum and at minimum should I use in each flask ?

For maximum - Check this protocol-online page - Maintenance of cell culture

For T-25 flask - maximum of 10 ml of medium.
For T-75 flask - maximum of 50 ml of medium.
For T-150 flask - maximum of 100 ml of medium.
For T-225 flask - maximum of 200 ml of medium.

### #3 Biog

Biog

Enthusiast

• Active Members
• 40 posts
2
Neutral

Posted 18 February 2009 - 02:36 PM

Thank you very much, "cellcounter" !

All the best

### #4 sciencedork

sciencedork

member

• Active Members
• 6 posts
0
Neutral

Posted 09 March 2009 - 01:02 PM

Okay...here is the proper way to do it...
calculation is as follows;
0.2 to 0.5 x cm2

This means that for a T25cm2 flask the volume range is 5-12.5mls for optimal CO2 penetration

calculation is done by multiplying 25 (for a T25cm2 flask) x 0.2 = 5ml lowest volume
25 x 0.5 = 12.5ml highest volume
75 x 0.2 = 15ml lowest volume
75 x 0.5 = 37.5ml highest volume and so on.

The measurements are gotten by measuring the base of the flask (or bottom). T75cm2 flasks are about 8cm x 9cm. You can even stand the flask up and use it that way. For a T75cm2 flask the bottom measures about 7.5cm x 3cm = 22.5cm2 = 4.5 to 11.2 ml volume. Get it? It really is based off a calculation that is imparitive for CO2 to penetrate the entire volume. Do not go by guessing or by what will and will not spill out of the flask. Hope this helps.

### #5 Biog

Biog

Enthusiast

• Active Members
• 40 posts
2
Neutral

Posted 08 May 2009 - 12:33 PM

Okay...here is the proper way to do it...
calculation is as follows;
0.2 to 0.5 x cm2

This means that for a T25cm2 flask the volume range is 5-12.5mls for optimal CO2 penetration

calculation is done by multiplying 25 (for a T25cm2 flask) x 0.2 = 5ml lowest volume
25 x 0.5 = 12.5ml highest volume
75 x 0.2 = 15ml lowest volume
75 x 0.5 = 37.5ml highest volume and so on.

The measurements are gotten by measuring the base of the flask (or bottom). T75cm2 flasks are about 8cm x 9cm. You can even stand the flask up and use it that way. For a T75cm2 flask the bottom measures about 7.5cm x 3cm = 22.5cm2 = 4.5 to 11.2 ml volume. Get it? It really is based off a calculation that is imparitive for CO2 to penetrate the entire volume. Do not go by guessing or by what will and will not spill out of the flask. Hope this helps.

OOOh ! I completely forget this !
Thanks a lot.
[[By the way, what the "T" stands for, in T25, T75... ? ]]