QuantiTect Primers with Roche SYBR
Posted 18 February 2009 - 07:20 AM
I need to run some gene expression assays fairly quickly and the QuantiTect Primer Assays available from Qiagen appear to be an easy way to get data fast. I can buy primers for my genes of interest and an internal control housekeeping gene without having to optimise We have a LC480 in the lab and a good stock of Roche SYBR green master mix, so I'm a bit reluctant to buy the equivalent from Qiagen for just a few experiments (in the long term I'd like to optimise and run everything using our Roche kits, etc).
My question is, has anyone ever tried using Qiagen primers and SYBR green master mix from another company? Qiagen say I won't have accurate expression if I do this, but will it really make that much of a difference?
Thanks in advance,
Posted 02 March 2009 - 10:54 AM
just now I searched for my gene of interest to give me primer sets, but it doesn't say what is inside the tube or doesn't say anything about the primer sequences.
I honestly don't understand why you buy Qiagen material for LC480. because Roche has its own kits ( I read you will optimize for Roche in the future, but still don't understand why). I think Roche has its own primer design and stuff too.
Posted 19 March 2009 - 08:58 AM
Hope that helps!