I'm working with mouse embryonic stem cells and I have encountered some trouble during EB formation. I just realized that I have been using the wrong kind of tissue culture dish for growing EB -- the one I was using are Corning treated culture dish (product #430167
) that are optimal for cell attachment. After differentiation, the cells grow on the bottom of the plate as a monolayer of cells instead of floating cell aggregates. I should really be using Non-treated culture dish or the Ultra-low attachment culture dish. I wanted to purchase those dishes but my lab manager said I can use the petri dish generally used for pouring agar plates (Fisherbrand, product #08-757-13
Can anyone tell me whether this type of petri dish can be used for culturing ES/EB? Has anyone ever used it for this purpose?
Also, I was wondering if I have over-trypsinized the ES cells off the feeders (I think I let that step go for ~5 minutes last time) that might have caused the failure of cell aggregation. Can someone please help me with this?
Thanks a bunch!
Edited by fakeopal, 17 February 2009 - 06:31 PM.