I get some bands not suppose to be there (indicated by red arrows). Since the templates I use are plasmids, there are not suppose to have non-specific bands.
The 2 plasmid construct difference in 1 exon that is the alternative splicing we are looking for, and those plasmids are used as positive control.
The cloudy band of 24+ has similar size of 24- that cause the problem to identify our sample result (indicated by blue arrow; I know it is very light but my boss say there is, so there is).
Can anyone help me get rid of those cloudy bands?
I notice that those cloudy bands begin to show up when we change agaorse for the PCR gel.
Will that be a problem?
By the way, my boss will use this agarose because it is much cheaper, so I cannot change agarose source for PCR gel.
Edited by wuxx0153, 17 February 2009 - 11:26 AM.