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phenol kristallin preparation


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3 replies to this topic

#1 anahid

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Posted 17 February 2009 - 07:13 AM

hi
Iwould like to know how I have to prepare phenol kristallin for DNA extraction?please help me.tanks

#2 bob1

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Posted 17 February 2009 - 03:33 PM

Go to the protocols page and click on the "media and solutions" link, scroll down to the link that says "phenol equilibration" and click on the "cached" link (the actual site is down) and follow the instructions there.

# Phenol Equilibration (Marcantonio Lab)
http://home.earthlin...protcls.html#...
Added: Tue May 14 2002, Hits: 330, Reviews: 0 Write review Cached

Be aware that phenol is quite toxic and is also a mild local anaesthetic so be very careful with large volumes.

#3 anahid

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Posted 18 February 2009 - 06:25 AM

Thanks bOb
this link is unavalibale.please tell me how can I equilibrate phenol and is it necessary to use hydroxyquinolin?

#4 mdfenko

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Posted 18 February 2009 - 08:55 AM

try this link: phenol search at vadlo

you can find a few methods.
talent does what it can
genius does what it must
i do what i get paid to do




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