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Seperate DNA spin columns


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3 replies to this topic

#1 D I Walker

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Posted 17 February 2009 - 04:36 AM

I am currently assessing cell lysis techniques that would be most suitable for my research. To do this I want to test lysis separately from purification. Because of this I need a standard DNA purification method which I can use when I change the lysis method I am testing.

I would like to use a DNA spin column as my purification standard. However so far I have had no luck finding any companies that sell the DNA spin columns separate from the lysis buffers in their kits.

Does anyone know where I can buy spin kits seperately? The only ones I have found so far have either been for RNA, to clean up PCR products or to extract DNA from agarose.

#2 Wolverena

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Posted 17 February 2009 - 07:52 AM

I am currently assessing cell lysis techniques that would be most suitable for my research. To do this I want to test lysis separately from purification. Because of this I need a standard DNA purification method which I can use when I change the lysis method I am testing.

I would like to use a DNA spin column as my purification standard. However so far I have had no luck finding any companies that sell the DNA spin columns separate from the lysis buffers in their kits.

Does anyone know where I can buy spin kits seperately? The only ones I have found so far have either been for RNA, to clean up PCR products or to extract DNA from agarose.


MOBIO (http://www.mobio.com/index.php) sells component separately from their kits. When you go to their website and look at their kits....go to the lower bottom right corner. There are the components of the kit, which most of the time can be purchased separately. For example, here are the kit components for the UltraClean DNA isolation kit:

http://www.mobio.com...tail.php?pid=90

Hope that helps.....Cheers!
"You can give somebody a book on 'How to ride bike' and then test that person on that knowledge. Even if that person gets an "A", it doesn't mean that he or she can ride a bike."
---this is what I am telling myself when I get a bad grade....as long as you don't loose your passion, you'll be fine.....V

#3 D I Walker

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Posted 19 February 2009 - 04:44 AM

Thanks for your reply. I have also found another company that sells spin columns seperately.

PeqLab (www.peqlab.co.uk)

#4 T C

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Posted 19 February 2009 - 06:06 AM

I think promega sells it as well.




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