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DNA quantification


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5 replies to this topic

#1 1stmolar

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Posted 17 February 2009 - 03:05 AM

Hi

I just received plasmid DNA from another institution which mentions 1.0 γ/λ as its conc. I am not familiar with that terminology. Can anybody help me with this?

Thanks.

#2 klinmed

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Posted 17 February 2009 - 04:01 AM

Hi

I just received plasmid DNA from another institution which mentions 1.0 γ/λ as its conc. I am not familiar with that terminology. Can anybody help me with this?

Thanks.


"Lambda" and "kappa" are easier to say than "microliter" and "microgram". These abbreviations have been used in labs for many years.

Your plasmid is at 1 g/L.

#3 phage434

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Posted 17 February 2009 - 08:45 AM

That's a gamma, not a kappa.

gamma = 1 microgram (Gram)
lambda = 1 microliter (Liter)

Only the real old timers use these any more. Respect them, they figured it out before you, with much, much worse tools!

#4 1stmolar

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Posted 17 February 2009 - 09:45 AM

Thanks guys.

#5 mdfenko

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Posted 18 February 2009 - 08:09 AM

Only the real old timers use these any more. Respect them, they figured it out before you, with much, much worse tools!

talk about the pot calling the kettle....

i still use "lambda", never really used "gamma" but am familiar with the terminology.

old habits...
talent does what it can
genius does what it must
i do what i get paid to do

#6 AquaPlasmid

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Posted 18 February 2009 - 09:31 AM

We always write this way on microfuge caps to save some room. Some even just write the numeric number to mean ug/ul. lol.




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