5 replies to this topic

[shimshady]

I ran out of the PCR clean up, spin through columns.  Are the plasmid miniprep spin through columns the same thing?  Are there any other protocols i can use that do not utilize the columns if they are not the same.  Thanks

Drew

[phage434]

They are the same.

[bob1]

They are similar, often the membranes on them are specifically designed with molecular weight cut-offs (higher and lower MW), so be careful about using plasmid ones with small PCR fragments.

[perneseblue]

note, you can easily reuse the spin columns. We have a very useful article on this here.

It does work.

[T C]

Hey

Like people have said, it can be used.  However, if you don't want to use the columns, you can always precipitate the DNA; the yields are generally higher but it takes more time.

TC

[molgen]

Try glycogen precipitation