Hi ,
I am trying to get the primers for the promoter region of usf1. I have picked the sequence in NCBi and pasted in meth primer... I got five options, but the problem is that the amplicons for M and U primers have the same numbers of pb e.g : 150 e 152 pb. How will I distinguish them in poliacrilamide gel???
Iam new in this subjet and iqam trying to find the way.
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#2
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Posted 18 February 2009 - 11:09 AM
It's OK if you would not mix up your M and U DNA templates. For example, do the M template PCR on day 1 and the U template on day 2. Or if you use methylight Real-time PCR with two taqman dyes. But I would try to pick another primer pair, just in case you might mislabel the tubes.
If you do fecal DNA hypermethylation study, please PM me and I can give you some idea of getting rid of the PCR inhibitors from your DNA preps.
If you do fecal DNA hypermethylation study, please PM me and I can give you some idea of getting rid of the PCR inhibitors from your DNA preps.
#3
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Posted 19 February 2009 - 12:34 PM
you would run the M and U primers in separte PCR reactions and then run them in different lanes on a gel, doesn't need to be PAGE.
Nick
Nick
