using the meth primer
Posted 16 February 2009 - 08:20 AM
I am trying to get the primers for the promoter region of usf1. I have picked the sequence in NCBi and pasted in meth primer... I got five options, but the problem is that the amplicons for M and U primers have the same numbers of pb e.g : 150 e 152 pb. How will I distinguish them in poliacrilamide gel???
Iam new in this subjet and iqam trying to find the way.
Posted 18 February 2009 - 11:09 AM
If you do fecal DNA hypermethylation study, please PM me and I can give you some idea of getting rid of the PCR inhibitors from your DNA preps.
Posted 19 February 2009 - 12:34 PM