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Has anyone had experience with GM2163 (dam-) cells?


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#1 rosepolaris

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Posted 16 February 2009 - 07:08 AM

Hi, guys, I got GM2163 cells from Fermentas and prepared competent cells. I transformed pCDF into the cells, however, I got many many colonies on control (without transformation). I have checked the product description from Fermentas and didn't see anything about antibiotics. I was thinking maybe my antibiotics (Str for pCDF) didn't work, so I picked up colonies from control to grow in fresh LB with new antibiotics. THe cells were still growing!

Now I am checking the cells from Fermentas, let them grow in fresh LB with antibiotics. Hopefully they will not grow..

If you have experience with dam- cells, please share with me. Did those cells work for you?

#2 rosepolaris

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Posted 16 February 2009 - 01:08 PM

Oh my, they are Str resistant ~~~!!!! Sorry to bother you all !!


Hi, guys, I got GM2163 cells from Fermentas and prepared competent cells. I transformed pCDF into the cells, however, I got many many colonies on control (without transformation). I have checked the product description from Fermentas and didn't see anything about antibiotics. I was thinking maybe my antibiotics (Str for pCDF) didn't work, so I picked up colonies from control to grow in fresh LB with new antibiotics. THe cells were still growing!

Now I am checking the cells from Fermentas, let them grow in fresh LB with antibiotics. Hopefully they will not grow..

If you have experience with dam- cells, please share with me. Did those cells work for you?






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