Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

RNA Extraction & EMSA


  • Please log in to reply
1 reply to this topic

#1 Mayflower

Mayflower

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 15 February 2009 - 10:57 AM



Hi,

I want to extract RNA from tissue culture cells for EMSA can anybody describe (or outline the steps) in brief the procedure from extraction to EMSA?


#2 molgen

molgen

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 167 posts
0
Neutral

Posted 16 February 2009 - 02:53 AM

I think that any extraction will do.
Once you have the RNA you have to mix in the protein and run it on a gel to see the shift.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.