Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Can we prepare Copmetent cell by calicum cloride with 0.09 OD?

  • Please log in to reply
No replies to this topic

#1 Dawood80



  • Members
  • Pip
  • 2 posts

Posted 15 February 2009 - 01:00 AM

:wacko: Hi All ,
I want to understand why we are allways preparing competent cell of Ecoli when we grow it till arrive OD into (0.5-0.6) ?
Because my experiment purpose need to very little cp cells ,So i can get a good result to my experiment when i prepare Competent cells with 0.09 optical density ,Is this OD. scientifically possible or not ?

Also due to my transformation result in broth media , i got declining in bacterial growth rate !!!! when the growth arrived to o.34 OD. but it has to continue increasing in bacteria growth like in Cp cell with 0.5 OD.
Any help please !!!!!!!!!!!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.