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Amaxa siRNA


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3 replies to this topic

#1 Wan

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Posted 13 February 2009 - 12:05 PM

I am using Amaxa kit to knock down a transcription factor expression in SK-N-SH cells, but got off-target effect.. Do you know anyway to fix this? I'm thinking if I cause apotosis.

#2 pcrman

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Posted 13 February 2009 - 11:21 PM

Do you mean using Amaxa nuclefection kit? How did you know it is off-target effect? If indeed the siRNA caused off target effects, you should redesign another siRNA targeting the same gene and do a thorough homology search.

#3 miRNA man

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Posted 16 February 2009 - 07:06 AM

Would recommend you try this tool (http://hollywood.mit.edu/targetrank/) and see if you're getting off target effects by miRNA-like binding.

#4 rajgene

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Posted 16 February 2009 - 09:31 AM

Amaxa transfection generates a degree of apoptosis in most of the cell types. did you include mock transfected control(cells+reagent alone) during each experiment..then you will know the %apoptosis due to the electrocution alone.
hope this helps.




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