E. coli Strains
Posted 13 February 2009 - 10:11 AM
I have been using a kit from Clontech called in-fusion 2.0 for cloning, produced my clones and transformed them into their "super competent" FusionBlue E. coli cells. This worked fine and produced many colonies, but I am keen to get back to using a more common generic cloning strain used in our lab already, or common in other labs.
I have tried to transform the same clones into DH5 alpha cells, but no colonies grew. For these reactions you need "super competent" cells (according to the website), with a transformation efficiency of greater than 10^8 cfu/ug DNA (that doesn't mean anything to me, but if it does to anyone else?....).
I was wondering if anyone could suggest any strains I could try, I've been told to try Invitrogen's Top10 E. coli or Excel-1-Blue/NovaBlue (Transformation efficiency: 10^9 cfu/μg DNA).
Are there any others which could be useful?
Posted 13 February 2009 - 11:50 AM
Posted 13 February 2009 - 08:45 PM
I use XL1 strain and it works very well. Infact for SDM they work better than the comp cells provided for the kit.
Posted 14 February 2009 - 08:30 AM
Posted 16 February 2009 - 09:02 AM