Difference between horse serum and FBS?
Posted 13 February 2009 - 09:24 AM
Posted 13 February 2009 - 11:53 AM
Honestly, I have no idea what happens if you add too much to your cells. I've never come across that before.
Too little will affect adherence (for an adherent line).. and given that logic, it would cause a attachment-free line to adhere.... other than that, I'm sorry. I don't know.
Posted 13 February 2009 - 05:57 PM
Adding too much serum to a culture could cause an imbalance in the electrolyte and growth factor concentrations in the solution, which will result in your cells not being happy, and possibly cause them to grow in unusual ways. It is best not to add too much serum to cultures, 20% is typically the maximum people add commonly.