could someone with a bit more experience than I have (I´m naive to this method yet) describe me the pros and cons of 1-step versus 2-step real time reverse transcription PCR. Meaning that when it would be better to do the reverse transcription separately and when in one and the same tube along with the PCR. The 1-step method is of course tempting but there must be some disadvantages too. Thank you, I appreciate the effort!
Edited by rajah, 13 February 2009 - 01:56 AM.