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How to wash ethanol away from tissue?


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#1 DocFlow

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Posted 13 February 2009 - 01:27 AM

I am going to extract DNA from pieces of fish fins that have been stored in ethanol. I guess I have to wash away as much ethanol as possible before extraction but has anybody any good protocol for this?
Is it as simple as incubating in 1xPBS for an hour or two or do I have to go through a more complicated and extended washing protocol? Thanks in advance

#2 cotchy

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Posted 13 February 2009 - 03:42 AM

If you pour off the liquid/supernatant and leave the fins to air dry in an eppendorf (in a laminar flow hood if you need sterility) the ethanol will evaporate pretty quickly, within about 30 mins i'd say if you remove most of the alcohol in the first step

Edited by cotchy, 13 February 2009 - 03:44 AM.


#3 T C

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Posted 13 February 2009 - 04:55 AM

Hey

Just to make sure that all the ethanol goes off, may be I would go for repeated washing with 1X PBS and vortex at every stage. Three times should be more than enough.

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TC

#4 DocFlow

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Posted 16 February 2009 - 04:25 AM

Hey

Just to make sure that all the ethanol goes off, may be I would go for repeated washing with 1X PBS and vortex at every stage. Three times should be more than enough.

Best
TC


Thanks to both of you. I guess I'll try with the PBS washing first, but simple air drying may work just as good. I'll have to try both. :)




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