cheap sybr green reagent
Posted 13 February 2009 - 12:48 AM
Teh sybr green from Invitrogen is too costly for my lab to afford these days. We're looking at alternatives and if half teh amount can be used instead of teh reommended 10ul of sybr green in a 20ul reaction?
Posted 13 February 2009 - 11:54 AM
That's $0.382/uL of 10,000x.
A 20 uL reaction containing 1x SYBR Green I costs $0.00076.
Or 10,000,000 uL (10 liters) of 1x SYBR Green I for $382.
That's a lot of PCR reactions. Can you split the costs with another lab?
Posted 13 February 2009 - 11:29 PM
2nd, you can get cheaper SYBR mix from alternative vendors such as from MCLab.com.