4 replies to this topic

[it_never_ends]

I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!

Thanks,

[jiro_killua]

it_never_ends, on Feb 12 2009, 02:50 PM, said:

I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!

Thanks,

The easiest way is to find collaborators who are using them

otherwise, you can also ask for generation of a gene trapped Dicer knockout from TIGM, either get the ES cells and make your own mice or have them do it for you, at about $15k, takes more than 1 yr

otherwise, if you want target knockout, there are other companies to make it for you, takes longer and more expensive

[Jon Moulton]

The faster/cheaper approach is a Dicer knockdown.

Wienholds E, Koudijs MJ, Van Eeden FJ, Cuppen E, Plasterk RH.  The microRNA-producing enzyme Dicer1 is essential for zebrafish development.  Nat Genet. 2003 Nov;35(3):217-8. Epub 2003 Oct 05.
Abstract:
http://www.nature.co...abs/ng1251.html

Place RF, Li LC, Pookot D, Noonan EJ, Dahiya R.  MicroRNA-373 induces expression of genes with complementary promoter sequences.  Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1608-13. Epub 2008 Jan 28.
Full text:
http://www.pubmedcen...bmedid=18227514

[cellcounter]

it_never_ends, on Feb 12 2009, 01:50 PM, said:

I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!

Thanks,

Looks like Dicer CKO mice are available from Jax, two strains.

[it_never_ends]

Cool!
Thanks everyone.