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Dicer-KO


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#1 it_never_ends

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Posted 12 February 2009 - 01:50 PM

I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!

Thanks,

#2 jiro_killua

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Posted 12 February 2009 - 05:07 PM

I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!

Thanks,



The easiest way is to find collaborators who are using them

otherwise, you can also ask for generation of a gene trapped Dicer knockout from TIGM, either get the ES cells and make your own mice or have them do it for you, at about $15k, takes more than 1 yr

otherwise, if you want target knockout, there are other companies to make it for you, takes longer and more expensive

#3 Jon Moulton

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Posted 13 February 2009 - 08:04 AM

The faster/cheaper approach is a Dicer knockdown.

Wienholds E, Koudijs MJ, Van Eeden FJ, Cuppen E, Plasterk RH. The microRNA-producing enzyme Dicer1 is essential for zebrafish development. Nat Genet. 2003 Nov;35(3):217-8. Epub 2003 Oct 05.
Abstract:
http://www.nature.co...abs/ng1251.html

Place RF, Li LC, Pookot D, Noonan EJ, Dahiya R. MicroRNA-373 induces expression of genes with complementary promoter sequences. Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1608-13. Epub 2008 Jan 28.
Full text:
http://www.pubmedcen...bmedid=18227514
Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com

#4 cellcounter

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Posted 13 February 2009 - 09:03 AM

I was just wondering if the Dicer Knockout cell lines/conditional KO mice were available commercially.. I know its been a while that people have been using them for, I just didn't know if you could get them somewhere!

Thanks,

Looks like Dicer CKO mice are available from Jax, two strains.

#5 it_never_ends

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Posted 16 February 2009 - 07:48 AM

Cool!
Thanks everyone.




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