PCR from a smear genomic DNA ?
Posted 12 February 2009 - 07:58 AM
the bacteria genomic DNA, somehow show some mild smearing in between the genomic band (upper part of gel) and RNA bands (RNA were not treated).
wonder is it okay to be PCR ? as i concern, since PCR only amplify the region i wanted, so the mild smearing part would not bring any effect onto it ? correct me if i am wrong.
Posted 12 February 2009 - 03:27 PM
Posted 12 February 2009 - 06:02 PM
Theoretically, there should be no effect.
Posted 15 February 2009 - 08:54 AM
Posted 15 February 2009 - 09:08 AM
apparently, i i got the band at desired size...
however, i was SHOCKED bcoz i noticed there was a band at very very upper part !
wat's that exactly ? it appears to me as genomic DNA band bcoz it was so huge that even beyond my ladder size (ladder used : Vivantis 100bp Plus ) . how could it be possible ? bcoz DNA get denatured over and over again, and only region that flanked by primers would be amplified, by the end of PCR, i shouldnt get this kind of big size DNA..
is that the contamination problem to you all ? correct me again if i am wrong. Thank you
Posted 15 February 2009 - 01:47 PM