I need to detect whether the transcription factors have already bound to DNA.
But the cells I use are very very few.
So I can't use EMSA to answere it.
Does anyone have the same problem?
Please give some advise to deal with it.
Questions about transcription factors
1 reply to this topic
Posted 11 January 2002 - 06:03 AM
you could use an in vivo footprint. Shorty fragmentate the DNA in cell culture, if a protein is bound you got protection. After this you make Adapter Ligation and PCRs and so can identify region where a protein has bound