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Questions about transcription factors


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#1 HS

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Posted 28 December 2001 - 05:10 PM

I need to detect whether the transcription factors have already bound to DNA.
But the cells I use are very very few.
So I can't use EMSA to answere it.
Does anyone have the same problem?
Please give some advise to deal with it.
Thanx!!

#2 DJ

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Posted 11 January 2002 - 06:03 AM

you could use an in vivo footprint. Shorty fragmentate the DNA in cell culture, if a protein is bound you got protection. After this you make Adapter Ligation and PCRs and so can identify region where a protein has bound




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