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glycine in unfolding buffer


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#1 methylation

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Posted 11 February 2009 - 02:19 PM

Hi all,

I'm unfolding my protein in buffer containing 50mM phosphate, 50mM glycine, 8M urea, and 1mM DTT. Could anyone explain the role of glycine in this buffer?

Thanks!

#2 mdfenko

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Posted 13 February 2009 - 06:56 AM

the glycine is a buffer that extends the useful range of the phosphate buffer with overlapping buffering ranges.
talent does what it can
genius does what it must
i do what i get paid to do




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