Hi all,
I'm unfolding my protein in buffer containing 50mM phosphate, 50mM glycine, 8M urea, and 1mM DTT. Could anyone explain the role of glycine in this buffer?
Thanks!
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an elder
Posted 13 February 2009 - 06:56 AM
the glycine is a buffer that extends the useful range of the phosphate buffer with overlapping buffering ranges.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
