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Glycine in unfolding buffer


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#1 methylation

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Posted 11 February 2009 - 02:17 PM

Hi all,

I'm unfolding my protein in buffer containing 50mM phosphate, 50mM glycine, 8M urea, and 1mM DTT. Could anyone explain the role of glycine in this buffer?

Thanks!

#2 mdfenko

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Posted 13 February 2009 - 07:29 AM

see my reply in your other posting (in the biochemistry forum). please don't post the same question in more than one forum.
talent does what it can
genius does what it must
i do what i get paid to do




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