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MOCK INFECTED CELLS


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4 replies to this topic

#1 bluebird

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Posted 11 February 2009 - 04:20 AM

Hi,

can anyone explain what Mock infected cells mean and give me some examples about materials to mock infection?

#2 little mouse

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Posted 11 February 2009 - 04:51 AM

It's when you infect cells with an empty vector. It's the control.

#3 bluebird

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Posted 11 February 2009 - 05:07 AM

It's when you infect cells with an empty vector. It's the control.


thanks yes it is for control but can you explain more. I'm new in this field. :blink:

#4 little mouse

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Posted 11 February 2009 - 05:35 AM

I guess that the infected cells are infected with a viral vector, to express a protein of interest, or to knockdown the expression of a protein. From this expression or knockdown of expression, you oberve a response. To be sure that the response is due to the expression of a protein, and not due to the infection itself, you infect control cells with the same viral vector but empty. It means it won't express the protein of interest.

#5 Curtis

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Posted 11 February 2009 - 09:27 AM

I asked the same question last week:

http://www.protocol-...?showtopic=6168




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