4 replies to this topic

[bluebird]

Hi,

can anyone explain what Mock infected cells mean and give me some examples about materials to mock infection?

[little mouse]

It's when you infect cells with an empty vector. It's the control.

[bluebird]

little mouse, on Feb 11 2009, 04:51 AM, said:

It's when you infect cells with an empty vector. It's the control.

thanks yes it is for control but can you explain more. I'm new in this field.

[little mouse]

I guess that the infected cells are infected with a viral vector, to express a protein of interest, or to knockdown the expression of a protein. From this expression or knockdown of expression, you oberve a response. To be sure that the response is due to the expression of a protein, and not due to the infection itself, you infect control cells with the same viral vector but empty. It means it won't express the protein of interest.

[Curtis]

I asked the same question last week:

http://www.protocol-...?showtopic=6168