Hi All!
Has anyone recently tested the new Zymo CpG Methylase Kit?
CpG Methylase (M.SssI)
They seem to have another buffer and definately a different incubation temperature. Any experiences regarding complete methylation?
Best,
MoB
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Posted 05 March 2009 - 11:57 PM
MoB, on Feb 11 2009, 01:01 PM, said:
Hi All!
Has anyone recently tested the new Zymo CpG Methylase Kit?
CpG Methylase (M.SssI)
They seem to have another buffer and definately a different incubation temperature. Any experiences regarding complete methylation?
Best,
MoB
Has anyone recently tested the new Zymo CpG Methylase Kit?
CpG Methylase (M.SssI)
They seem to have another buffer and definately a different incubation temperature. Any experiences regarding complete methylation?
Best,
MoB
Hi All!
I give feedback on my own posting. I tested the Zymo CpG Methylase Kit after convincing my lab-leader that it should be worth to try. We had some problems receiving a complete methylation with the NEB M.SssI kit. With the Zymo kit methylation seems to be complete even for extremely CpG-rich regions for which the NEB kit had some difficulties. In all cases we weren't able to detect any residual unmethylated DNA.
The main differences are a different buffer and overnight-incubation at 30°C (NEB 37°C, 1-4h). Zymo ships their kit on dry-ice. Shipment by NEB is done on cool-packs. This seems to have a high impact on the stability especially of the SAM. This is the component we have identified to be most critical. Any comments on that?
Best,
MoB
