Solvent for Agar
Posted 11 February 2009 - 02:45 AM
i wish to quantify the biomass growing on an agar plate. to do this i want to liquify the agar either with heat or with a suitable solvent and pull it through a filter, leaving the biomass on the filter membrane, which i can then weigh.
obviously i dont want to damage my cells such that they fall through the filter.
has anyone had any experience with doing this? whats a good solvent for dissolving agar with little heat?
any help will be appreciated
Posted 11 February 2009 - 04:11 AM
The fungus grows on the membrane but still gets all nutrients from the medium. Then you take the fungal biomass with the filter/membrane off your plate, dry it and determine the biomass. You should not forget to write down the weight of your membrane before putting it on the agar plate, it has to be subtracted from your biomass
Edited by gebirgsziege, 11 February 2009 - 04:13 AM.
Posted 12 February 2009 - 04:17 AM
Posted 13 February 2009 - 11:46 AM
I don't know what it might do to your cells.
Posted 13 February 2009 - 02:27 PM
Posted 13 February 2009 - 03:05 PM
If you only want to know the amount of biomass generated, you have to scrap/ rinse to get most of them off the plate and spin it to collect biomass that way.
Posted 14 February 2009 - 05:21 AM
Failing that - the application of enzyme is not justified. In any case, geberg's sugg is much better.