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preparation of SSC


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#1 bluebird

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Posted 11 February 2009 - 12:37 AM

Hi,

I want to prepare SSC(standard sodium citrate) can anyone help me with the froumula.

#2 scolix

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Posted 11 February 2009 - 12:53 AM

Hi,

I want to prepare SSC(standard sodium citrate) can anyone help me with the froumula.


Have you check in Maniatis, the lab manual for molecular biology.

#3 scolix

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Posted 11 February 2009 - 12:55 AM

Try this one from this site: http://openwetware.o...emicals#20x_SSC

20x SSC

Recipe to make 4L

* Dissolve 701.28g NaCl and 352.92g NaCitrate
* pH to 7.0
* Bring final volume to 4L
* Autoclave if necessary

#4 bluebird

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Posted 11 February 2009 - 01:48 AM

Many thanks for help :blink: B)




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