condensation on agar plates
Posted 10 February 2009 - 05:56 PM
I'm having trouble cloning and recently condensation from the lid dropped inside the agar plate as I was plating my transformed cells. I didn't get any colonies. Thus would the condensation have caused any problem and how should I avoid it in teh future. I do store my pates upside down but just before plateing, I turn themteh right side up for 10mins.
Posted 10 February 2009 - 06:13 PM
The lid of the plate is lifted just high enough to allow the plate to be poured, and the dish is quickly half filled with agar. #The lid is REPLACED, the plate swirled gently to ensure even distribution of the molten agar, then left to stand on the bench for at least 20 minutes to solidify. The plates are then stored upside down in fridge.
I do see condensation after teh plates have solidified and just store them upside down in the fridge. The condensation on the plates after teh agar solidifies and just before I store the plates in the fridge, a problem?
Posted 10 February 2009 - 07:22 PM
Posted 11 February 2009 - 12:49 AM
But the condensation should not affect colony growth. There is some problem with your ligation or transformation.
Posted 11 February 2009 - 01:04 AM
Two other problems can be caused by condenstation: 1. that the plate is "sealed" by a water film between the lid and the bottom of the plate. But this problem does not occur when cloning....you are not incubating your cells long enough that they use all the air.
2. it drops on your plate and your microbes begin to mix as they are spread all over the plate by the water film....
Therefore: carefully prepare your plates and let them dry....for tips on agar plates see here
Posted 11 February 2009 - 05:05 AM
This allows the agar to cool sufficiently that when it is poured no condensation will appear (or only on the top plates of a stack of them) as the heat coming off them is not high enough.
Posted 13 February 2009 - 08:56 AM