I'm double digestion my vector (working well)
Purifying it on a gel
Excising the appropriate band (get lovely bands)
Normally the next step would to be do a gel extraction but for some reason the kits are giving me very very low yields (almost nothing). Have decided to maybe go back to basics and try a phenol-chloroform extraction and see what the problem is.
My question to you is, since my sample is purely DNA, can I skip the phenol-chloroform part and simply jump straight to the ethanol precipitation? Do I just dissolve my excised band in water and proceed with the precipitation or do I need to dissolve my sample in a particular buffer to avoid interference from the agarose?
Many many thanks for anyone able to help, really very much appreciate it guys.
Edited by NiceButDim, 10 February 2009 - 12:59 PM.