Posted 06 February 2009 - 06:09 PM
Also if I forgot to briefly rinse my membrane after blocking and I just directly added my diluted Ab will this affect the results significantly?
I blocked the membrane with the same blocking agent that I dilute my Ab in. So i thought this should be ok.
Has anybody done the same mistake as I did? and still got good results?
Posted 07 February 2009 - 07:05 AM
Posted 11 February 2009 - 07:50 AM
I have used it and was not very happy with it...tried 0,5 - 5 % fish gelatine in TBST...BSA 5 % in TBST was much better
Posted 30 March 2010 - 02:26 AM
I guess, when you use the same solution for blocking and for dilution of your Ab, it doesn't matter if you wash it beetwen or not.